Fermentation organisms : a laboratory handbook / by Alb. Klöcker; translated from the german by G. E. Allan and J. H. Millar.

Date:
1903
Catalogue details

Licence: In copyright

Credit: Fermentation organisms : a laboratory handbook / by Alb. Klöcker; translated from the german by G. E. Allan and J. H. Millar. Source: Wellcome Collection.

Provider: This material has been provided by the Royal College of Physicians of Edinburgh. The original may be consulted at the Royal College of Physicians of Edinburgh.

    121/458 (page 97)
    and the operator ought not to consider himself efficient until he can keep flasks containing yeast water or meat extracts sterile after repeated manipulations. Experiments with Solid Culture Media—If a portion of a o’rowth is to be inoculated from a solid culture medium o into a flask containing nutrient solution or on to new solid medium, then metal wires, such as platinum, or glass rods are used. Before use they are sterilised in the flame and placed in a flamed tin box, which is covered with a glass plate likewise flamed. They are left in the box until quite cool. This is very important, as one might otherwise easily kill the growth to be introduced. Immedi- ately before use they are once again drawn quickly through the flame. When glass rods are to be used it will be better in many cases to take one drawn out into a long thin point, especially when small specks of growth {e.g., from a moist chamber) or a fine mycelium, which clings easily to inoculation needles when these are used, has to be introduced, as the point of the rod can be broken ofl* and left in the infected culture liquid. The infection is thus performed more surely and more quickly than when a metal wire has to be rubbed against the sides of the Hask in order to leave particles of growth in the liquid. Sometimes small pieces of platinum wire are used for the same purpose, being manipulated by a pair of forceps. Infection of a solid culture medium takes place either on or below its surface. A surface culture is laid on most easily as a streak made with a metal wire or glass rod; if the culture to be introduced is contained in a liquid, a pipette may be used, by means of which we then sow a drop on the surface of the solid culture medium ; or we may use a metal loop. On the other hand, if the culture is to be sown in the substratum, this may be performed by aid of a metal wire (an inoculation needle), which with the 7