Fermentation organisms : a laboratory handbook / by Alb. Klöcker; translated from the german by G. E. Allan and J. H. Millar.

Date:
1903
Catalogue details

Licence: In copyright

Credit: Fermentation organisms : a laboratory handbook / by Alb. Klöcker; translated from the german by G. E. Allan and J. H. Millar. Source: Wellcome Collection.

Provider: This material has been provided by the Royal College of Physicians of Edinburgh. The original may be consulted at the Royal College of Physicians of Edinburgh.

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    by means of fish glue and then the ring fastened to the glass slip by means of vaseline. The ring must be adjusted on the glass slip so that the adherent medium is not dis- solved by the water. In most cases only one drop of water is placed in the middle of the chamber, so that even when the adherent substance is soluble in water, there will be little chance for the ring to become loose. It is certainly best to use an insoluble medium when a loose adhesion with vaseline is not employed. An adhesive medium can be made from a mixture of wax and a little turpentine ; it is painted on after melting. Syndetikon, fish glue, water glass, etc., are all soluble in water. Two sizes of rings are in general use, having diameters of 30 mm. and 18 mm. respectively. The former are used chiefly for preparing pure cultures, where the presence of a large number of colonies is desired in one chamber. Squared cover Fig. 43.—Stand for Moist glasses are often used for this. Stand for Moist Chambers.— When man}^ moist chambers with cultures are in use at the same time it is convenient to have a stand for them (see Fig. 43). 9.—Additional Aijparatus. Pipettes are used in many sizes and it is necessary to have a large stock of them. Some of these must be gradu- ated and have a capacity of | c.c. to 100 c.c. It is also necessary to have ready various sizes of ungraduated pipettes, a number of them being drawn out at one end into long capillary tubes. Some of each kind must have the lower part long and thin enough to reach to the bottom of the Pasteur flasks through the side tubes. Such pipettes