Serum diagnosis of syphilis and the butyric acid test for syphilis / by Hideyo Noguchi.

Date:
[1911]
Catalogue details

Licence: In copyright

Credit: Serum diagnosis of syphilis and the butyric acid test for syphilis / by Hideyo Noguchi. Source: Wellcome Collection.

Provider: This material has been provided by the Royal College of Physicians of Edinburgh. The original may be consulted at the Royal College of Physicians of Edinburgh.

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    X. TECHNIC OF THE WASSERMANN SYSTEM. In performing the test by the original Wasser- mann system five different factors are required, viz.: antigen, patient's sermn, complement, amboceptor, and blood-corpuscles. The source and mode of preparation of these factors will be given in detail below. PREPARATION OF ANTIGEN. A. AQUEOUS EXTRACTS. 1. Wassermann's Method.—The liver or spleen of a congenitaUy syphHitic foetus is preferred. Take the organ and cut it up into very small pieces with a pair of scissors and mix the tissue with four parts of physiological salt solution to which phenol in the proportion of 0.5 per cent, is added. For example: 860.0 c.c. salt solution (0.85 per cent.) 100.0 grams liver 40.0 c.c. phenol (5 per cent.) This mixture is thoroughly shaken in a dark bottle for twenty-four hours by means of a shaking machine. The tissue pieces are separated by centrifugalization and the brownish, opalescent supernatant fluid is used for antigen. It should be preserved in a rubber- stoppered dark flask in the refrigerator. Upon