Strategy of the viral genome / edited by G.E.W. Wolstenholme and Maeve O'Connor.

  • Symposium on Strategy of the Viral Genome (1971 : London, England)
Date:
1971
Catalogue details

Licence: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

Credit: Strategy of the viral genome / edited by G.E.W. Wolstenholme and Maeve O'Connor. Source: Wellcome Collection.

    118/432 (page 104)
    104 david baltimore et ul. and Baltimore 1968). As can be seen in Fig. 2, the profile of proteins separated on Polyacrylamide gels in the presence of sodium dodecyl sulphate (SDS) shows two very large proteins (NC VP 00 and NC VP 0) made in the presence of amino acid analogues which are not found in their absence. The larger of these, NC VP 00, has a molecular weight of 200 000-300 ооо, which is the size expected for a total transcript of the poliovirus genome. Fraction No. Fig. 2. Polyacrylamide gel electrophoresis of polypeptides synthesized in infected cells either (a) in the absence or (b) in the presence of four amino acid analogues. Reproduced from Jacobson, Asso and Baltimore (1970) by permission of the publisher. A second type of experiment also shows the existence of NCVP 00. Cells were infected at 37°С with poliovirus and after three hours the temperature was raised to 43°С and radioactive leucine was added. Comparison of the protein labelled under these conditions with the protein made in cells maintained at 37°C showed that both NCVP о and NCVP 00 were made at the higher temperature (Fig. 30). Fig. shows that when