Strategy of the viral genome / edited by G.E.W. Wolstenholme and Maeve O'Connor.

Symposium on Strategy of the Viral Genome (1971 : London, England)

Date:: 1971

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Licence: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

Credit: Strategy of the viral genome / edited by G.E.W. Wolstenholme and Maeve O'Connor. Source: Wellcome Collection.

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DISCUSSION 71 REFERENCES Atkinson, M. R., Hubeeman, J. A., Kelly, R. B. and Kornberg, A. {1969) Fedtt Proc. Fedn Am. Socs exp. Biol. 28, 347 (abstract no. 503). Bishop, D. M. L., Mills, D. R. and Spiegelman, S. (1968) Biochemistry, 7, 3744-3753. Doi, R. H. and Spiegelman, S. (1962) Science 138,1270-1272. Glacomoni, D. and Spiegelman, S. (1962) Science 138,1328-1331. Goodman, H. M. and Rich, A. (1962) Proc. natu. Acad. Sci. U.S.A. 48, 2101-2109. Hall, B. D. and Spiegelman, S. (1961) Proc. natn. Acad. Sci. U.S.A. 47,137-146. Haruna, I., Nozu, K., Ohtaka, Y., and Spiegelman, S. (1963) Proc. natn. Acad. Sci. U.S.A. 50, 905-911. Haruna, I. and Spiegelman, S. (1965) Science 150, 884-886. Levisohn, R. and Spiegelman, S. (1968) Proc. natn. Acad. Sci. U.S.A. 60, 866-872, Levisohn, R. and Spiegelman, S. (1969) Proc. natn. Acad. Sci. U.S.A. 63, 805-811. Mills, D., Pace, N. and Spiegelman, S. (1966) Proc. natn. Acad. Sci. U.S.A. 56,1778-1785. Mills, D. R., Peterson, R. L. and Spiegelman, S. (1967) Proc. natn. Acad. Sci. U.S.A. 58, 217-224. OvERBY, L. R., Barlow, G. H., Doi, R. H., Jacob, M. and Spiegelman, S. (19660) J. Bact. 91,442-448. OvERBY, L. R., Barlow, G. H., Doi, R. H., Jacob, M. and Spiegelman, S. {1966b) J. Bact. 92, 739-745. Pace, N. R. and Spiegelman, S. (19660) Proc. natn. Acad. Sci. U.S.A. 55,1608-1615. Pace, N. R. and Spiegelman, S. (1966b) Science 153, 64-67. Saffhill, R., Schneider-Bernloehr, H., Orgel, L. E. and Spiegelman, S. (1970) J. motee. Biol. 51, 531-539. Spiegelman, S., Haruna, I., Holland, I. В., Beaudreau, G. and Mills, D. (1965) Proc. natn. Acad. Sci. U.S.A. 54, 919. Watanabe, I. (1964) Nihon Rinsho 22,1187-1195. Yankofsky, S. a. and Spiegelman, S. (19620) Proc. natn. Acad. Sci. U.S.A. 48,1069-1078. Yankofsky, S. a. and Spiegelman, S. (19626) Proc. natn. Acad. Sci. U.S.A. 48,1466-1472. DISCUSSION Qß POLYMERASE AND MUTANT TEMPLATES in vitVO Subak-Sharpe: Obviously the molecule should be able to incorporate deoxyribonucleotides. But can you by judicious selection eventually make a DNA molecule without any ribonucleotide ? Spiegelman: We can make mutants that will tolerate incorporation of deoxynucleotides and apparently force the enzyme to do it for them. These are selected under very limiting concentrations of one of the riboside triphosphates while this is being supplemented with the corres¬ ponding deoxynucleoside triphosphate. It would be very amusing to push it to the very end of the game, but we have not done so yet. Littauer: What is the mechanism for selecting a given molecule ? Spiegelman: I don't think it is any different from any other mutations. We are getting a number of things here, and once we get down to the limiting size the only thing to do is to change bases. How those mistakes