Experimental investigation into the disease, produced by the inoculation of the bacillus pyocyaneus / by M. Armand Ruffer.

  • Ruffer, Sir Marc Armand, 1859-1917.
Date:
1889
Catalogue details

Licence: Public Domain Mark

Credit: Experimental investigation into the disease, produced by the inoculation of the bacillus pyocyaneus / by M. Armand Ruffer. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    c, Enteritis. d, Albuminuria. Wasting. /, Haemorrhages. In one word, these chemical substances reproduce all the symptoms, and most of the pathological appearances to which the bacillus itself gave rise when inoculated. Let us see now whether these substances are also found in the tissues of infected animals. IL The blood is naturally the first fluid which ought to have been examined for these poisons, and it is their presence in that fluid which will be studied here first. It is only fair to state however, that Professor Bouchard's researches on the presence of vaccinating substances in the urine had preceded our own on the blood. The following method was used to demonstate the pre- sence of vaccinating substances in the blood of infected animals. One or two rabbits were inoculated with a cul- ture of the living bacillus pyocyaneus in the afternoon of one day, and the next morning, that is about eighteen hours after the inoculation, these animals always ex- hibited the characteristic signs of the pyocyanic disease. The animals were then tied down on the table and bled to death from the jugular vein, or from the femoral artery. An equal quantity of water was added to the blood thus collected (from 40 to looccs.), and this mix-